How blocking Raf activates the MAPK pathway
نویسندگان
چکیده
منابع مشابه
RAF inhibitors activate the MAPK pathway by relieving inhibitory autophosphorylation.
ATP competitive inhibitors of the BRAF(V600E) oncogene paradoxically activate downstream signaling in cells bearing wild-type BRAF (BRAF(WT)). In this study, we investigate the biochemical mechanism of wild-type RAF (RAF(WT)) activation by multiple catalytic inhibitors using kinetic analysis of purified BRAF(V600E) and RAF(WT) enzymes. We show that activation of RAF(WT) is ATP dependent and dir...
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The dynamic balance between polymerization and depolymerization of microtubules is critical for cells to enter and exit mitosis, and drugs that disrupt this balance, such as taxol, colchicine, and nocodazole, arrest the cell cycle in mitosis. Although the Raf/MEK/MAPK pathway can be activated by these drugs, its role in mitosis has not been addressed. Here, we characterize activation of Raf/MEK...
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ژورنال
عنوان ژورنال: Pigment Cell & Melanoma Research
سال: 2010
ISSN: 1755-1471
DOI: 10.1111/j.1755-148x.2010.00680.x